Obtaining cattle embryos using ready-made media of the scientific and production enterprise "Paneco"

Abstract

The development and widespread practical application of in vitro embryo production technology will make it possible to replenish the herd population in a short time, preserve and replicate valuable genotypes, as well as create new genotypes using the method of genome editing. Livestock breeding is tasked with providing agriculture with a high–value herd in a fairly short time, while farmers are charged with replenishing the herd in a short time, which is possible only with the active introduction of assisted reproductive technologies, such as in vitro embryo production (IVP technology). The use of domestically produced cultural media in technology will ensure greater accessibility, reduced economic costs and the rapid development of breeding workin Russia. The purpose of the work was to evaluate the effectiveness of the IVP technology when using culture media from the domestic manufacturer SPE “PanEco” (Russia) to obtain cattle embryos in vitro. Our own protocol for obtaining cattle embryos was based on the protocols of culture media manufacturers SPE “PanEco” and IVF Bioscience (UK). Thus, for the first time using the line of media from the domestic manufacturer “PanEco” to obtain cattle embryos in vitro under incubation conditions at temperatures from plus 38.6 ◦C to plus 38.9 ◦C, CO2 content 6.2-6.8 % and uncontrolled humidity, on 6-8 days after a period of time from 140 to 182 hours from the moment of fertilization, 30.4 % of embryos were obtained at the blastocyst stage in relation to the total number of fertilized oocytes that reached the stage of 2 – 4 blastomeres.