Obtaining and determining antioxidant activity of polyphenolic extracts obtained from propolis

Abstract

Over the years, time has given us a whole series of novelties in the fi eld of natural medicine, which have managed to truly prove their value and keep up to date. Th e application of apitherapy has been known since ancient times and today the interest in this branch of natural medicine is growing worldwide. Among bee products, with an indisputable value is also propolis, which is currently widely studied, and as a result is the subject of multiple therapeutic uses based on evidence. Research in the fi eld of propolis preparations has expanded considerably and is of major medical and pharmaceutical interest. Propolis is an excellent anti-infl ammatory, healing and epithelializing, hepatoprotective, immuno-biostimulator, capillary tonic and tissue regenerator. It is remarkable that, due to its complexity and compositional variability, propolis retains its full antimicrobial capacity, unlike synthetic antibiotics, which bacteria develop resistance over time, requiring the periodic introduction of new antibiotics. Th e study on obtaining and analyzing propolis extracts, by determining the total polyphenolic and antioxidant activity, by scavenging method of the free radical DPPH, is an important premise for the use and development of new pharmaceutical forms containing propolis for multiple curative-prophylactic uses based on local raw materials.