Abstract
The SARS-CoV-2 virus is a new coronavirus that caused the worldwide pandemic of the human respiratory disease COVID-19. To detect the SARS-CoV-2 virus in environmental objects and in clinical samples, molecular genetic (PCR), immunological (ELISA, IHA), virological research methods are used. The purpose of this study was to obtain a strain-producer of the recombinant protein Spike_RBD, with an introduced hex histidine label, for its subsequent development, chromatographic purification and use in the development of experimental kits for the immunological diagnosis of COVID-19 in humans and to assess the intensity of the cellular and humoral immune response to the SARS-CoV-2 virus. To achieve the goal, the following tasks were set: 1) to create a genetic construct for the expression of Spike RBD antigen in Escherichia coli cells; 2) to obtain a Spike RBD antigen producer strain; 3) to evaluate the effectiveness of Spike RBD antigen expression by a producer strain. The relevance of the work is related to the production and use of recombinant RBD antigen of the SARS-CoV-2 virus, as well as to the development of diagnostic test systems.
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