Abstract
1. 1. Apparatus is described for the disruption of micro-organisms by ultrasonic radiation. It is based on a magnetostriction transducer operating at 26 kc/sec and a power of 500 W. 2. 2. The length of the stainless steel cup containing the material under irradiation is a critical variable. Maximum breakage of baker's yeast occurs with tube lengths of 10.5 and 18.5 cm and minimum breakage with tube lengths of 12.5 and 20.5 cm. 3. 3. The degree of cell breakage is an inverse function of the volume of the suspension irradiated, a negative exponential function of the time of irradiation and relatively independent of the cell concentration. 4. 4. Making certain assumptions, a relationship is deduced between the fraction of cells remaining intact ( R), the volume of the suspension ( V) and the time of irradiation ( t) which fits reasonably well with the experimental findings. This relationship has the form log R=−constant× t/ V.
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