Abstract
This work presents the dependence of the electrochemical (EC) detection of catechol and indole-related compounds on the pH and organic solvent composition of the mobile phase as well as on the column temperature, including a performance comparison with the UV detection. Particular attention has been paid to the effect of the above chromatographic conditions on the peak shape and peak area recorded by two detection modes. It was found that EC detector gave significantly worse peak shape than UV detector for some derivatives in certain eluents. Furthermore, chromatographic parameters and especially mobile phase pH influenced oxidation of these compounds much more than their UV absorbance. As a result, it was proved that the selectivity and flexibility of the EC detector can be varied not only by variation of electrochemical conditions but also by variation of the mobile phase pH taking into account the excess peak tailing obtained for some basic solutes at near neutral pH.
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