Observations on the genetic structure and mating system of ponderosa pine in the Colorado front range

Abstract

Variation of peroxidase enzymes is analyzed both in mature needle tissue and in open-pollinated seedling families of ponderosa pine, Pinus ponderosa, and is identified as being controlled by a single Mendelian locus. Variation at this locus, analyzed in 1, 386 individuals, is used in the analysis of population differentiation and the mating system. Significant variation of gene frequencies is detected over distances of several hundred meters, and is found to be associated with slopes of different aspects. Ponderosa pine is wind-pollinated, and an analysis of the mating system indicates that the level of outcrossing is greater than 90 %. Selection specific for different environments is evidently strong enough to overcome the homogenizing force of migration and produce population fissuring in ponderosa pine.