Abstract
The development of a method to measure small amounts of nucleotides would be of considerable value in experiments concerned with metabolism in mammalian spermatozoa. As the adenine nucleotides have been shown to be the predominant nucleotides in mammalian spermatozoa (Brooks, 1970), measurement of ATP and ADP by the luciferin-luciferase system appeared to be a method ideally suited to this purpose. Ejaculated bull semen, collected by artificial vagina, was used in the present work. The measurement of ATP was carried out in a Packard Model 3380 liquid scintillation spectrometer calibrated according to Stanley & Williams (1969). The phosphate buffer described by these workers was altered to include 2 mm-EDTA and 2 mm-2-mercaptoethanol. In addition, the luciferin-luciferase preparation made up in arsenate buffer was incubated with apyrase to reduce background luminescence
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
