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Abstract
The amphibian virus, LT-1, produced relatively irregular necrotic areas in the monolayer of rainbow trout ovarian cells. The infected cells underwent the following cytopathic changes: extensive cytoplasmic vacuolation, formation of multiple nuclei, and paranuclear cytoplasmic inclusions.The site of replication of the virus in the infected rainbow trout ovarian cells was demonstrated to be located at the less electron-dense areas of the cytoplasm and surrounded by numerous vacuoles. A comparison of the susceptibility to LT-1 virus of rainbow trout ovarian cells with that of fathead minnow cells resulted in a calculated cytopathic index of 4.48.
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