Observations on human ova and the fertilization process

Abstract

The mammalian fertilization process was reviewed, with emphasis on recent laboratory developments. Maturation stages of the human follicular oocyte which are requisite to fertilization were explored with in vitro culture methods. The experience of other investigators was confirmed in that, on removal from the follicle, nuclear maturation through polar body release was observed. Available information on the human tubal ovum was summarized and the recorded attempts at in vitro fertilization of the human oocyte analyzed. Time lapse in cinematography used successfully in the rabbit was suggested as a means for establishing the validity of in vitro fertilization in man. It was predicted, based on experience in other mammals, that placement of in vitro cultured human oocytes into the uterus for implantation would eventually be carried out successfully. Further studies in the subhuman primate were suggested as a requisite to such experiments, in order to insure conditions which would yield normal young.