Abstract

The behavior of small solid bodies in contact with two immiscible liquids has been much discussed, especially in connection with phagocytosis. Direct observation of interfacial phenomena is possible with bacteria or other particles in films viewed in the darkfield microscope. A small drop of each phase, one of them containing the suspended bacteria, is placed on a clean slide. A cover slip is laid on top. The boundary between the two liquids appears as a brilliant band and the bacteria as shining motes. Bacteria in two phase films of water with a variety of organic liquids have been studied.Ordinary Gram-positive or Gram-negative bacteria have been trapped in the liquid-liquid interface of all preparations examined. The trapping mechanism is much stronger in preparations with high (e. g., hydrocarbon-water) than with low (e. g., alcohol-water) liquid-liquid interfacial tension. Bacteria in the interface exhibit characteristic gliding movements along the interface, moving in the boundary line from regions ...

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