Observation on the effect of breviscapine on glomeruli in mice with cisplatin-induced renal damage

Abstract

Objective To observe the protective effect of breviscapine on the glomerulus and glomerular basement membrane in mice with cisplatin-induced renal damage. Methods Thirty-two C57BL/6J mice were randomly divided into a blank control group, a model group, a 25 mg breviscapine experimental group, and a 50 mg breviscapine experimental group. Except for the blank control group, the other 3 groups were intraperitoneally injected cisplatin 8 mg•kg-1 to prepare cisplatin renal damage model in mice. For the 2 experimental groups, gavage was performed in 25 mg•kg-1 and 50 mg•kg-1 breviscapine for 10 d, then the mice were sacrificed. HE and PAS staining methods were used to observe the pathological changes of glomerular and make a score. Western Bloting method was used to detect the expressions of collagen type Ⅳ (Col-Ⅳ), fibronectin (FN), and laminin (LN). Results Compared with the blank control group, the model group showed increased number of glomerular cells, volume, and glomerular sclerosis, adhesion of Bowman's capsule (P < 0.05, P < 0.01). The expressions of Col-IV, FN, and LN had obvious gene deletion (P < 0.01). In the low dose and high dose of breviscapine in the experimental groups, the renal damage was relieved and the integral glomerular pathological indicators were significantly lower than those in the model group. The expressions of Col-IV, FN, and LN increased significantly in the glomerular basement membrane compared the experimental groups with the model group (P < 0.05, P < 0.01). Conclusion Breviscapine has a good protective effect on cisplatin-induced glomerular damage in mice and a certain repair effect on glomerular basement membrane damage. Key words: Breviscapine; Cisplatin; Renal damage; Glomeruli; Basilar membrane