Abstract
Observation on the Changes in Lactate Dehydrogenase Isoenzymes in Post-Burn Patients: Significance in Relation to Creatine Kinase The present study deals with the quantitative assessment of lactate dehydrogenase isoenzymes in the sera of burn subjects. Efforts are also made here to show better predictive marker value of sera LDH, as a few other known protein markers like creatine kinase and myoglobin have limited analytical value in the management of thermal burns. Blood was initially collected at day-1 of admission from 29 burn and 10 healthy subjects. Further, the sampling was carried out at 2, 5, 10, 20 and 30 days of wound healing (recovery). Plasma and sera LDH isoenzymes were monitored on 7.5% polyacrylamide gel electrophoresis. Quantitative assessment of LDH isoenzymes was done from gelscans using GelPro and Scion Imaging softwares. Sera CK levels were estimated colorimetrically using reagent kits. Our results show that quantitative changes in LDH isoenzyme were more convincing and interpretable in the sera than plasma. Sera LDH-5 isoenzyme was detected as the major contributor of total sera LDH activity, which follows a change parallel to sera CK in burn subjects. Sera LDH-5 activity also remains significantly high for up to 10 days while sera CK levels were detected elevated up to 5 days (P<0.05) during the recovery of patients. Therefore, the present findings strongly recommended the use of sera to assess the LDH activity and indicate better stability of sera LDH-5 than sera CK during post burn wound healing.
Highlights
Proteins and enzymes or their multiple forms are fairly reliable markers to assess the type and extent of damage to tissues under clinical conditions
Plasma and sera lactate dehydrogenase (LDH) isoenzymes were monitored on 7.5% polyacrylamide gel electrophoresis
Our results show that quantitative changes in LDH isoenzyme were more convincing and interpretable in the sera than plasma
Summary
Proteins and enzymes or their multiple forms are fairly reliable markers to assess the type and extent of damage to tissues under clinical conditions. 1.1.1.27.) is Abbreviations: lactate dehydrogenase (LDH); creatine kinase (CK); polyacrylamide gel electrophoresis (PAGE). Human LDH resolves into five isoenzyme bands. The activity levels of individual isoenzyme bands are determined by the physiological function and metabolic or clinical state of affected tissue. Tissue-specific normal isoenzyme profiles of humans are found disturbed in a number of clinical conditions and carry diagnostic value. A few published reports have dealt with LDH activity leakage in the sera of burn patients [15,16]. Despite the vast body of published evidence available on other clinical conditions, it is intriguing that the comparative value of LDH isoenzymes in burn subjects has not been fully evaluated
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