Abstract
Objective To study the immunogenicity of Toxoplasma gondii excreted/secreted antigens (ESA) and soluble tachyzoite antigen (STAg) after intranasal immunization. Methods BALB/c mice were randomly divided into 3 groups, 10 mice each group. Mice were intranasally immunized with 20 μl PBS per mouse and 20 μg ESA or STAg per mouse, respectively, twice at an internal of 2 weeks. Ten mice of each group were killed on day 14 after the last immunization. Spleen lymphocytes and intestinal intraepithelial lymphocytes (iIEL) were counted. Serum IgG and IgA in intestinal washes were detected by ELISA. Results Cell proliferation of spleen lymphocytes and iIEL was observed in all antigen groups on day 14 after the last immunization, and compared with the PBS group, there was a significant difference ( P ＜0.05). The level of serum IgG in all antigen groups was higher than that in PBS group on day 14 after immunization with a significant difference ( P ＜0.01 ). On day 14 after immunization, the level of IgA in ESA group and STAg group was higher than that in PBS group (P ＜ 0.01 ). Conclusion Intranasal immunized with ESA or STAg can effectively induce the mucosal and systemic immune responses with potent immunogenicity. Key words: Toxoplasma gondii; Excreted/secreted antigen; Soluble tachyzoite antigen; Intranasal immunization; Immunogenicity
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
