Abstract
Introduction: Guttate is a type of psoriasis in which patients are sensitive to Streptococcus pneumoniae throughout innate immune responses. During the inflammation, tumour necrosis factor alpha (TNF-α), a well-known pro-inflammatory cytokine, is expressed; meanwhile interleukin 10 (IL-10) and heparin-binding EGF-like growth factor (HB-EGF), which are capable of inhibiting transcription of the TNF-α gene, are also prominent. Furthermore, HB-EGF only impacts fibroblasts and keratinocytes which promote psoriatic lesions. In this study, we looked for differences of TNF-α, IL-10 and HB-EGF expression between a psoriatic patient and a non-psoriatic relative. Methods: To achieve our target, peripheral blood mononuclear cells (PBMCs) expressing LPS receptors or CD14 (CD14+ cells) derived from a guttate patient, and the donor’s father (without psoriatic symptoms), were activated for 7 days by a lysate of Streptococcus pneumoniae for 24 hours before being harvested. Results: Results showed detectable mRNAs of TNF-α, IL-10 and HB-EGF from isolated CD14+ cells of guttate patient were more intensive expression than the non-psoriatic one at 24 hours after engaging the bacterial components. In addition, transcription of HB-EGF gene from the guttate patient was maintained over 168 hours, while its mRNA level from the non-psoriatic volunteer was only expressed within 24 hours. Conclusion: Finally, in initial results of inflammatory effects between strains, the Streptococcal lysate was seen to have stronger immune responses than the Staphylococcal lysate on the immune cells of the guttate psoriasis.
Highlights
Guttate is a type of psoriasis in which patients are sensitive to Streptococcus pneumoniae throughout innate immune responses
To monitor innate immune response of CD14+ cells from a patient with guttate psoriasis in vitro, the streptococcal lysate was used for this purpose
FBS was not supplemented into the RPMI 1640 medium because it contains several elements which could overlap with the transcriptional effects of heparin-binding epidermal growth factors (EGFs)-like growth factor (HB-EGF)
Summary
Guttate is a type of psoriasis in which patients are sensitive to Streptococcus pneumoniae throughout innate immune responses. The vast majority of these loci encode major histocompatibility complex (MHC), pro-inflammatory cytokines and nuclear transcriptional factors in both innate and adaptive immune responses (Jordan et al, 2012; Nair et al, 2006; Sato et al, 2015). Those elements are abnormally increased in gene expression in active peripheral blood mononuclear cells. A streptococcal infection in the throat could lead to an exacerbation of almost all lesions on psoriasis patients during inflammation (Gudjonsson et al, 2003) According to these results, forming a psoriatic lesion might require environmental stimulators to activate cytokines involved in immune responses. Psoriasis might be a consequence of imbalance of inflammatory homeostasis
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