Abstract

This study focused on applying a patented medium-chain fatty acids (MCFAs) mixture at the end of alcoholic fermentation and monitoring its residues. MCFAs are a promising agent that has the potential to increase the efficiency of sulfur dioxide and ultimately minimize its doses, which is one of the important goals of wine research today. Detailed octanoic, decanoic, and dodecanoic acid contents were observed during the experiment. The MCFA mixture was applied at doses of 0, 10, 20, and 60 mg/L. GC–MS determined the content of individual fatty acids. The results showed that the use of the investigated mixture of fatty acids at doses of 10 and 20 mg/L did not cause an increase in the content of individual fatty acids residues. The octanoic acid content after application of the 20 mg/L MCFA mixture was 8.24 mg/L after 744 h, while the untreated control variant showed a value of 7.71 mg/L. The performed sensory analysis also did not show a negative effect of MCFA application on the sensory properties of wine. Therefore, applying an MCFA mixture at 10 and 20 mg/L can be recommended as a safe alternative following alcoholic fermentation. However, the results obtained can also serve as a valuable basis for permitting the use of MCFA in the proceeding OIV approval process. The research thus opens the possibility of expanding a new oenological agent capable of reducing SO2 doses.

Highlights

  • Examples of medium-chain fatty acids (MCFAs) in wines are hexanoic, octanoic. and decanoic acid. Their negative effect on the fermentation of clear musts has been known for a long time

  • It can be stated that the application of the investigated mixture of MCFA at doses of 10 and 20 mg/L did not cause an increase in the content of individual fatty acids in comparison with the control variant

  • The content of octanoic acid at 744 h after application was statistically identical to the control variant in these variants

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Summary

Introduction

Sulphur dioxide is one of the most common additives in wine technology. It is used to terminate alcoholic fermentation and as an antioxidant effect during the ageing and storage of wines. One alternative to the use of SO2 may be applying medium-chain fatty acids (MCFAs) These are compounds naturally formed in wine during alcoholic fermentation [3]. The presence of octanoic acid and especially decanoic acid lowers the intracellular pH in yeast cells and increases the permeability of the plasma membrane [12] to an effective form of molecular SO2 [13]. This effect can be used to reduce SO2 doses at Published: 28 February 2022. The experimental process has shown the effectiveness of applying a mixture of C8, C10, and C12 fatty acids following alcoholic fermentation [14] Based on these results, a mixture of C8:C10:C12 was patented in the ratio 2:7:1 [17].

Design of Experiment
Sensory Analysis
Determination of the Fatty Acids by GC-MS
Determination of Glucose and Fructose Content
Preparation of the MCFA Mixture Solution
Results and Discussion
Decanoic Acid
Dodecanoic Acid
Glucose + Fructose
Results
Conclusions
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