Abstract
Antioxidant activity and phenolic compound was found in Justicia gendarussa via total phenolic content (TPC) and ,-diphenyl--pycrilhydrazil hydrate (DPPH) radical scavenging assays. The assays were applied on aqueous and methanolic extracts of leaves, callus culture and cell suspension culture of J. gendarussa . Callus was induced from green and matured leaves of J. gendarussa c ultured on Murashige and Skoog (MS) basal medium supplemented with different concentrations of 2,4- dichlorophenoxyacetic acid (2,4-D) or 1-naphthaleneacetic acid (NAA) and benzylaminopurine (BAP). Callus induced on NAA showed higher phenolic content and antioxidant activity as compared to the one induced on 2,4-D. The highest total phenolic content (88 mg GAE/g) was observed in callus culture induced with NAA and BAP, while the highest antioxidant activity (lowest IC 50 ; 8.46 × 10 -3 mg/ml) was observed in the cell suspension. Key words: Anti-oxidant, callus, Justicia gendarusa , suspension culture.
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