Abstract
We studied the properties of highly concentrated hemoglobin solutions from 137 to 290 mg/mL using small angle neutron scattering over a very large momentum transfer range from q = 3.4 × 10−4 to 0.16 Å−1. The 137 and 227 mg/mL solutions revealed the presence of a large-scale superstructure with a radius of gyration on the order of 3000 Å. The 290 mg/mL solution was characterized by a mass fractal dimension of 2.67. Additionally, the scattering curve of the 290 mg/mL solution exhibited two shoulders, which might be due to the shape of the superstructure. In contrast, the radius of gyration of a single hemoglobin molecule is ∼24 Å.
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