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Abstract
Nowadays, researchers are focusing on sorting, characterizing and detecting micron or submicron particles or bacteria in microfluidic chips. However, some contradictions hinder the applications of conventional microfluidic chips, including the low working distance of high resolving power microscopy and the low light transmittance of conventional microfluidic chips. In this paper, a rapid and readily accessible microfluidic fabrication method is presented to realize observation with high magnification microscopy. With the one-step molding process, the interconnections, the thin observation interface of polydimethylsiloxane (PDMS) membrane and microfluidic channels were integrated into an intact PDMS replica. Three kinds of PDMS replicas with different auxiliary beams were designed and optimized by leakage experiments and analytical software. The observation interfaces of a 170 μm thickness PDMS membrane enlarges the application domain of microfluidic chips. By adopting a solution of high magnification observation, microfluidic devices could be applied widely in medical science, biology and material science.
Highlights
Microfluidic devices have an array of important applications in areas such as analytical systems [1,2], biomedical devices [3], material science [4], chemistry tools and biochemistry [5]
In the work described here, we present a one-step molding technique that allows the rapid and efficient construction of microfluidic chips with observation interfaces of thin PDMS membrane for efficient construction of microfluidic chips with observation interfaces of thin PDMS membrane for high magnification light microscopy observation and detection
With less than 300 μm of PDMS membrane, PDMS replicas were compatible with most commercial high magnification lens
Summary
Microfluidic devices have an array of important applications in areas such as analytical systems [1,2], biomedical devices [3], material science [4], chemistry tools and biochemistry [5]. The contradiction between the thickness of microfluidic chips and the working distance (W.D.) of high-resolution microscopy prevents PDMS microfluidic devices from being further exploited in many applications. In addition to looking forward to the development of microscopy devices, microfluidic techniques techniques are essential in meeting the needs of the observation and detection of submicron are essential in meeting the needs of the observation and detection of submicron particles. Perhaps thin and flat PDMS membranes have the potential for high-magnification observation and observation and precise detection of submicron particles and bacteria in microfluidic channels. In the work described here, we present a one-step molding technique that allows the rapid and efficient construction of microfluidic chips with observation interfaces of thin PDMS membrane for efficient construction of microfluidic chips with observation interfaces of thin PDMS membrane for high magnification light microscopy observation and detection.
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