Obligate Anaerobic Bacteria in the Gastrointestinal Microflora of the Grass Carp (Ctenopharyngodon idella), Goldfish (Carassius auratus), and Rainbow Trout (Salmo gairdneri)

Abstract

Grass carp (Ctenopharyngodon idella), bred in Arkansas and maintained in British Columbia under defined culture conditions on pelleted diets and on aquatic weeds, were examined to determine their commensal gastrointestinal bacterial flora. Using anaerobic incubation, the total bacterial numbers cultured ranged from 6 × 104 to 4 × 108 (average 4 × 106) per g of alimentary tract plus contents. Obligately anaerobic bacteria were isolated and identified as species of Actinomyces, Bacteroides, Eubacterium, Fusobacterium, and Peptostreptococcus. This is the first description of the presence of these strictly anaerobic organisms in the gastrointestinal tract of fish. Indeed, many of the anaerobes isolated did not conform to previously described species. Members of the Genus Clostridium were also isolated by enrichment culture and appeared to be associated with a pond weed diet. A wide variety of facultative anaerobic bacteria were also isolated, with Aeromonas hydrophila predominating. The gastrointestinal tracts of goldfish (Carassius auratus) were also found to contain sizable numbers of obligate anaerobes, but the gastrointestinal tracts of hatchery-cultured rainbow trout (Salmo gairdneri) contained insignificant numbers. Key words: grass carp, anaerobic bacteria, gastrointestinal microflora, Aeromonas hydrophila, rainbow trout, goldfish