Abstract
The present study aimed to identify key metabolites related to weight reduction in humans by studying the metabolic profiles of sera obtained from 34 participants who underwent dietary intervention with black soybean peptides (BSP) for 12 weeks. This research is a sequel to our previous work in which the effects of BSP on BMI and blood composition of lipid were investigated. Sera of the study were subjected to ultra performance liquid chromatography and quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS), and the data were analyzed using partial least-squares discriminate analysis (PLS-DA) score plots. Body mass index and percent body fat of the test group were reduced. Levels of betaine, benzoic acid, pyroglutamic acid, pipecolic acid, N-phenylacetamide, uric acid, l-aspartyl-l-phenylalanine, and lysophosphatidyl cholines (lysoPCs) (C18:1, C18:2, C20:1, and C20:4) showed significant increases. Levels of l-proline, valine, l-leucine/isoleucine, hypoxanthine, glutamine, l-methionine, phenylpyruvic acid, several carnitine derivatives, and lysoPCs (C14:0, PC16:0, C15:0, C16:0, C17:1, C18:0, and C22:0) were significantly decreased. In particular, lysoPC 16:0 with a VIP value of 12.02 is esteemed to be the most important metabolite for evaluating the differences between the 2 serum samples. Our result confirmed weight-lowering effects of BSP, accompanied by favorable changes in metabolites in the subjects' blood. Therefore, this research enables us to better understand obesity and increases the predictability of the obesity-related risk by studying metabolites present in the blood.
Highlights
Obesity is considered a perilous disease because, besides being widespread, it is the primary risk factor for the development of various physical, mental, and social disorders such as cardiovascular diseases, type 2 diabetes, obstructive sleep apnea, certain types of cancers, mental depression, and social stigmatization [1, 2]
Soh et al tried to identify the weight-reducing effects of fermented soybean products, chungkukjang and doenjang, by analyzing the hepatic mRNA expressions of enzymes related to fatty oxidation [4, 5]
We reported that obese research subjects who had taken black soybean peptides (BSP) supplementations experienced significant reduction in body weight, body mass index (BMI), and body fat mass
Summary
Obesity is considered a perilous disease because, besides being widespread, it is the primary risk factor for the development of various physical, mental, and social disorders such as cardiovascular diseases, type 2 diabetes, obstructive sleep apnea, certain types of cancers, mental depression, and social stigmatization [1, 2]. Certain Korean traditional fermented soybean foods; fermented soybean and fermented soybean pastes, notably Chungkukjang and Doenjang, may help to alleviate or fight obesity [4,5,6]. Soh et al tried to identify the weight-reducing effects of fermented soybean products, chungkukjang and doenjang, by analyzing the hepatic mRNA expressions of enzymes related to fatty oxidation [4, 5]. Most of these research results are unable to correlate substances responsible for antiobesity as soybean and soybean products contain numerous compounds with functional properties, for example, saponins, isoflavones, and proteins [7,8,9,10]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
