Obesity-Related Changes in Human Plasma Lipidome Determined by the Lipidyzer Platform.

Péter Pikó,Zsigmond Kósa,Róza Ádány,Sándor Szűcs,László Pál,János Sándor

doi:10.3390/biom11020326

Abstract

Obesity is an increasing public health concern both in the developed and developing countries. Previous studies have demonstrated that considerable alterations in lipid metabolism and consequently marked changes in lipid profile are associated with the onset and progression of obesity-related complications. To characterize the full spectrum of obesity-induced changes in lipid metabolism, direct infusion tandem mass spectrometry analysis is the most promising approach. To better understand which of the many lipid species are the most strongly associated with obesity, the aim of our work was to measure and profile plasma lipids in normal (n = 57), overweight (n = 31), and obese (n = 48) individuals randomly selected from samples of Hungarian general and Roma populations by using the targeted quantitative lipidomics platform, the Lipidyzer. Principal component and stepwise regression analyses were used to identify the most significant clusters and species of lipids by increasing body mass index (BMI). From the 18 clusters identified four key lipid species (PE P-16:0/20:3, TG 20:4_33:1, TG 22:6_36:4, TG 18:3_33:0) showed a strong significant positive and three others (Hex-Cer 18:1;O2/22:0, LPC 18:2, PC 18:1_18:1) significant negative association with BMI. Compared to individual lipid species alone, the lipid species ratio (LSR) we introduced showed an extremely strong, at least 9 orders of magnitude stronger, association with BMI. The LSR can be used as a sensitive and predictive indicator to monitor obesity-related alterations in human plasma and control the effectiveness of treatment of obesity associated non-communicable diseases.

Highlights

Obesity is an increasing public health concern both in the developed and developing countries [1,2]
Clusters showing positive relationships with body mass index (BMI) included 307 lipid species belonging to the classes of CEs, DGs, PCs, PEs, and triacylglycerol species (TGs) and clusters presenting negative association with BMI comprised 12 lipid species from PCs, PEs, LPCs, and LPEs lipid classes
Lipid molecules demonstrating positive relationships with BMI included 27 lipid species belonging to the classes of CEs, PCs, PEs, TGs, and

Summary

Introduction

Obesity is an increasing public health concern both in the developed and developing countries [1,2]. The latest available data from the World Health Organization (WHO), from 2016, showed that 1.9 billion adults were overweight of which 650 million obese worldwide [2]. It can play a pivotal role in the development of several chronic pathological conditions and diseases including insulin resistance, type-2 diabetes, cardiovascular diseases, musculoskeletal disorders, fatty liver disease, and cancer [1,2]. Previous studies have demonstrated that obesity is not an increase in the mass of body adipose tissue, but it is associated with considerable alterations in different metabolic pathways, among them in lipid homeostasis [4]. The altered concentrations of plasma lipids such as cholesterol, triacylglycerols, and low- and high-density lipoproteins

Objectives

Results

Conclusion