Abstract
WNIN/Obese (WNIN/Ob) rat a new mutant model of metabolic syndrome was identified in 1996 from an inbred Wistar rat strain, WNIN. So far several papers are published on this model highlighting its physical, biochemical and metabolic traits. WNIN/Ob is leptin resistant with unaltered leptin or its receptor coding sequences - the two well-known candidate genes for obesity. Genotyping analysis of F2 progeny (raised from WNIN/Ob × Fisher - 344) in the present study localized the mutation to a recombinant region of 14.15cM on chromosome 5. This was further corroborated by QTL analysis for body weight, which narrowed this region to 4.43 cM with flanking markers D5Rat256 & D5Wox37. Interval mapping of body weight QTL shows that the LOD score peak maps upstream of leptin receptor and shows an additive effect suggesting this as a novel mutation and signifying the model as a valuable resource for studies on obesity and metabolic syndrome.
Highlights
Obesity, referred to as overweight due to excess fat accumulation has assumed epidemiological proportion in the last decade [1,2] along with its associated disorders [3,4,5,6]
During 1990’s, a ‘Fat’ rat with about 47% of its body weight representing fat was identified from this stock, and later by pedigree and back cross analysis, a mutant strain was established as a uni-locus model for obesity trait, designated as WNIN/Ob
Hypertriglyceridemia, hypercholesterolemia and hyperleptinemia, it turned out to be a worthwhile model for metabolic syndrome, with accelerated ageing and degenerative disorders like impaired immunity, tumours, infertility, polycystic ovaries, cataract and retinal degeneration [18,19,20,21]
Summary
Obesity, referred to as overweight due to excess fat accumulation has assumed epidemiological proportion in the last decade [1,2] along with its associated disorders [3,4,5,6]. To determine association between the marker and bodyweight phenotype, single factor analysis of variance was conducted on body weights of F2 progeny rats with ‘A’ genotype versus ‘H’ genotype. Phenotype, and map distance data (estimated using ONEMAP package) were imported into R/QTL.
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