Oat rhizogenesis due to the effects of phytohormones

Abstract

Purpose. Select the optimum concentration of phytohormones and investigate their effect on oat rooting and develop a nutrient medium for induction of rhizogenesis in vitro.Methods. For rooting, cloned material of different breeding samples of oats No. 493-27 was used; No. 477-5; No. 399-38; No. 425-19 and the varieties of Decameron and Gift. Clone rooting was performed on nutrient media according to Murasige and Skug and Hamburg and Eveleg with reduced macrosolids, which served as control variants, and for experimental variants used auxins (IOC, NOC, IMC) and cytokinins (6-BAP, zept, and gibberellins (HA) in concentrations of 0.1 to 1.5 mg/l. During the growth and development of oat plants recorded the beginning of root formation, the presence of callus formation, the percentage of rooted plants (%), the number of roots (pieces), the length of the root system (see), plant growth (see) and their overall condition.Results. In breeding variants, on average, at a concentration of IBC of 1.0 mg/l, the percentage of rooted oats was 72.9 %, and in the varieties of this indicator was slightly higher – 81.6 %. With the use of NOC in the specified concentration in the breeding samples, the rooting index was 88.6 % and in the varieties – 89.5 %. The highest percentage of rooting was established with the addition of IOC in varietal samples – 89.7 % and 92.3 % in varieties. In the varieties Decameron and Gift, the length of the oat root system was longer compared to the linear material compared to the linear material. КаDecameron for use of all auxins and the gift from IOC and IMC had a root system length – 7.0 cm and NOC – 5.0 cm. Linear materials had a root system length using NOC – 4.5 cm, and in IOC – 5.7 cm, IMC – 5,2 cm. The number of roots in oat plants with auxin addition varied from 2,0 pc. up to 3,8 pcs. At concentrations of gibberellic acid – 1.0 mg/l oat plants had a height of 18 to 26 cm, and the length of the root system ranged from 12 to 25 cm.Conclusions. A nutrient medium has been developed that allows to obtain 91.0 % of rooted oats for 14 days. Rhizogenesis is advisable to carry out on the environment of Hamburg and Eweleg with the addition of auxins. The addition of gibberellic acid to the nutrient medium, regardless of concentration, provided a significant lengthening of the oat root system and the extraction of leaves and internodes. The highest percentage of rooted plants was observed in the combination of IOC and NOC, and the lowest in the use of IBS in all variants. The use of auxin concentrations greater than 1.0 mg/l was inappropriate, since the inhibition of the external state of the plants and the increase in the percentage of rooted plants did not occur.