Abstract
Methods Two novel models of the adult human foreskin mucosa were established: 1) ex vivo polarized inner and outer foreskin explants; 2) in vitro immuno-competent reconstructed foreskins, which include fibrous sheets produced by foreskin fibroblasts that are topped by differentiating foreskin keratinocytes (from either inner or outer foreskin) and Langerhans cells (LCs). These models permit to inoculate comparatively in a polarized manner at the mucosal pole either HIV-1-infected cells (that are present in all secretions vectorizing HIV-1) or cell-free HIV-1.
Highlights
Male circumcision was reported to reduce male acquisition of human immunodeficiency virus type 1 (HIV-1) by 60%, the initial mechanisms of HIV-1 transmission at the male genitals remain elusive
Two novel models of the adult human foreskin mucosa were established: 1) ex vivo polarized inner and outer foreskin explants; 2) in vitro immuno-competent reconstructed foreskins, which include fibrous sheets produced by foreskin fibroblasts that are topped by differentiating foreskin keratinocytes and Langerhans cells (LCs)
LCs have an active role in sampling HIV-1 at the foreskin followed by transfer of virus to T-cells
Summary
Address: 1Cellular Biology and Host-Pathogen Interactions, Cochin Institute, Paris, France, 2Anatomy & Pathological Cytology Service, GH Cochin-St Vincent de Paul, Paris, France, 3Urology Service, Necker Hospital, Paris, France, 4Geoffroy St Hillaire Clinic, Paris, France and 5Reproductive Biology Service, GH Cochin-St Vincent de Paul, Paris, France. Published: 22 October 2009 Retrovirology 2009, 6(Suppl 3):O7 doi:10.1186/1742-4690-6-S3-O7. AIDS Vaccine 2009 Anna Laura Ross Meeting abstracts – A single PDF containing all abstracts in this Supplement is available here. http://www.biomedcentral.com/content/pdf/1471-2105-10-S12-info.pdf
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
