O3: EVALUATION OF A 3D PRINTED BIO-ARTIFICIAL MUSCLE-ORGANOID FOR TRAUMA RESEARCH

Abstract

Abstract Introduction Organoid models serve as a robust platform for investigating injury and disease in vitro. Currently, representative models of injury are lacking to investigate the effects of traumatic damage to organs and tissues. Here we describe a three-dimensional in vitro model of high strain rate loading seen in traumatic blast injury. Method 3D printing resins were tested for Young's Modulus & Poisson Ratio using a universal testing Organoids were then loaded into a 3D Printed bioreactor for high strain rate loading using a split Hopkinson pressure bar device. machine and digital image. Euler beam theory was used to evaluate post deflection. C2C12 myoblasts were seeded in fibrin hydrogels around 3D printed posts using a custom designed jig. High strain rate loading was applied to constructs, then qPCR & Fluorescent Live/Dead staining was utilised to demonstrate cell alignment and myotube formation. Result Young's modulus of Flexible resin was 11.51Mpa. Differentiated C2C12 myoblasts were capable of alignment between posts and expression of key markers of differentiation shown by qPCR & imaging. MYH5, MYH2 & MYH1 all had a > 1.5 old increase in expression compared to undifferentiated controls. Organoids were capable of survival in bioreactor casings for over 24 hours and were intact after application of high strain rate loading. Conclusion This work demonstrates the first use of a 3D printed organoid in vitro model to investigate high strain rate loading for trauma research. This organoid is capable of high throughput analysis to facilitate genomic and protein level expression analysis. Take-home message This work demonstrates the first use of a 3D printed organoid in vitro model to investigate high strain rate loading for trauma research.