O2-9-2 - Pancreatic Cancer Patient-Derived Orthotopic Xenograft (Pdox) Cured by Fluorescence-Guided Surgery Followed by Uvc

Abstract

Background: The aim of this study is to determine if ultraviolet light (UVC) irradiation in combination with fluorescence- guided surgery (FGS) can eradicate metastatic human pancreatic cancer in orthotopic nude–mouse models.Methods: Two weeks after orthotopic implantation of human MiaPaCa-2 pancreatic cancer cells, expressing green fluorescent protein (GFP), in nude mice, bright-light surgery (BLS) was performed on all tumor-bearing mice (n = 24). After BLS, mice were randomized into 3 treatment groups; BLS-only (n = 8) or FGS-only (n = 8) or FGS-UVC (n = 8). The residual tumors were resected using a hand-held portable imaging system under fluorescence navigation in mice treated with FGS and FGS-UVC. The surgical resection bed was irradiated with 2700 J/m2 UVC (254 nm) in the mice treated with FGS-UVC.Results: The average residual tumor area after FGS only (n = 16) was significantly smaller than after BLS only (n = 24) (0.135±0.137 mm2 and 3.338±2.929 mm2, respectively; p = 0.007). The BLS-only treated mice had significantly reduced survival compared to FGS- and FGS-UVC treated mice for both relapse-free survival (RFS) (p < 0.001 and p < 0.001, respectively) and overall survival (OS) (p < 0.001 and p < 0.001, respectively). FGS-UVC-treated mice had increased RFS and OS compared to FGS-only treated mice (p = 0.008 and p = 0.025, respectively), with RFS lasting at least 150 days indicating the animals were cured.Conclusion: The results of the present study suggest that UVC irradiation in combination with FGS has clinical potential to increase survival.