Abstract
High plasma homocysteine (Hcy) levels may indicate cardiovascular disease. However, sensitive and selective determination of Hcy remains a major challenge. Herein, we present a sensing strategy for Hcy by surface enhanced Raman scattering (SERS) method along with a specific reaction of o-phthalaldehyde (OPA) and Hcy. The obtained adduct 2-(1-carboxyl-3-thiopropyl)-1-isoindolinone (Hcy-OPA) can be directly detected by SERS using gold nanoparticles (Au NPs) as the substrate. The developed SERS method displays superior sensitivity (low detection limit of 2.50 × 10−12 mol L−1) with a broad linear range (5.00 × 10−10 −5.00 × 10−6 mol L−1). As a proof of real application, it can be used to detect Hcy in bovine serum samples with a concentration as low as 5.00 × 10−9 mol L−1, which is free from the interference of the other amino acids and glutathione.
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