Abstract
Serum was obtained from 25 male renal cell carcinoma (RCC) patients and 21 healthy males. O-glycans were released by a β-elimination reaction and purified by graphitized carbon cartridge solid phase extraction, then profiled by matrix-assisted laser desorption/ionisation-time of flight mass spectrometry. After noise removal and peak alignment, 1372 peaks were extracted from 200000 data points. Feature peaks were analyzed by calculation of differential sensitivity and specificity. The combination of two feature peaks was chosen as a biomarker and could clearly differentiate RCC and normal samples in our study group.
Highlights
Serum was obtained from 25 male renal cell carcinoma (RCC) patients and 21 healthy males
The combination of two feature peaks was chosen as a biomarker and could clearly differentiate RCC and normal samples in our study group
Because matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) offers a simple means for screening complex mixtures [17], it plays a crucial role in the characterization of glycosylation [18]
Summary
Serum was obtained from 25 male renal cell carcinoma (RCC) patients and 21 healthy males. The combination of two feature peaks was chosen as a biomarker and could clearly differentiate RCC and normal samples in our study group. Local RCC incidence increased from 0.4 per 1000 in 2000 to 0.6 in 2010 [3], and has become an important threat to health in China. Glycans attached to proteins have very important functions in cancer biology. No. easier to identify and quantify compared with proteins because of their smaller molecular weight and greater stability [9]. All these factors make glycans appropriate potential biomarkers [10] for cancer
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