O.30 Arginine-enriched total enteral nutrition increaseliver albumin gene expression and does not increase iNOS expression

Abstract

liver albumin gene expression and does not increase iNOS expression J. Ren, S. Wang, Y. Zhao, J. Li and A. Li Research Institute of General Surgery, Jingling Hospital, Nanjing University, Nanjing, China. Aim: To examine the effects of arginine enriched total enteral nutrition (TEN) on liver albumin, TNF, IL-lcq IL-6R and inducible nitric oxide synthase (iNOS) gene expression. Methods: A 30% burn was applied in 40 rats after 7 days of gastrostomy. The rats received a continuous intragastric infusion of two different diets 2 hours after burn: low arginine (AL, arginine 0.01 g/kg/day) and arginine enriched (AE, arginine 0.264 g/kg/day). The two formulas were isocaloric (1 75 Kcal/kg/day) and isonitrogenous (1.25 gN/kg/day). The animals were sacrificed on day 1 post burn (PBD1) and PBD9. The mRNA of albumin, TNF, IL-l(z, IL-6R and iNOS of the liver were reverse transcribed (RT) into cDNA and the cDNA were amplifed by polymerase chain reaction (PCR). The RT-PCR products of albumin, TNF, IL-lc~, IL-6R and iNOS were determined by capillary electrophoresis (CE) and semi-quantified by the specific peak area of the CE chromatogram. Results: RT-PCR products of albumin, TNF, IL-6R and iNOS (absorbance unit). Albumin TNF IL-6R iNOS AL(PBD1) 869_*:52 1031 _+23 4320_+108 965-+41 AE(PBD1) 813_+34 984+_27 3810+_217 984_+39 AL(PBD9) 1417 -+ 43 ~ 743 _+ 29* 2570 -+ 90* 547 _+ 23* AE(PBDg) 1812_+41** 603_+32** 972-+87 **~: 522_+48* Means -+ SD, t-test: * P < 0.05 vs AL(PBD9), ** P < 0.01 vs AL(PBD9), 1: P < 0.01 vs PBDI.