Abstract
Abstract Understanding human peri-implantation development is necessary to improve in vitro fertilisation. Technical progress, such as scRNAseq and integrated stem cell models have dramatically improved our ability to understand human peri-implantation development. However, in-depth analysis and systematic validation in human embryos remains necessary to build a reliable reference. Here, we started by integrating pre- and post-implantation scRNAseq embryo datasets to generate a peri-implantation reference, highlighting progression of fates during early human development. Second, we identified the dynamics of genes modules, groups of genes with similar expression pattern. In particular, this highlighted the hierarchy of transcription factor potentially driving cell fate progression. Importantly, we performed prolonged embryo culture to validate by immunofluorescence progression of EPI and TE fates. Moreover, we compared the expression profile of the identified markers in stem cell models. Finally, we used the combination of gene modules to benchmark blastoid models. Altogether, our study paves the way for modelling human peri-implantation development with stem cell models. Trial registration number NA Study funding: Funding source:
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