Nutritional quality of phloem sap in relation to host plant-alternation in the bird cherry-oat aphid

Abstract

Host alternation in aphids has been attributed to complementary growth of host plants, or more specifically to seasonal changes in the nitrogen quality of the phloem sap. In this report, seasonal fluctuation of free amino acids in phloem of the winter and summer host plants (Prunus padus, bird cherry and Hordeum vulgare, barley) of Rhopalosiphum padi (the bird cherry-oat aphid) were investigated in the context of aphid growth and behaviour. Phloem was collected from the cut stylets of aphids taken from plants that were grown outdoors. The total concentration of amino acids in P. padus phloem increased between bud break and late flush (spring), decreased in mature leaves (summer), and increased again in early senescent leaves (autumn). In H. vulgare, however, amino acid concentration fluctuated less from seedlings to flowering. Spring aphids from P. padus grew rapidly on this host from bud break to late flush, but died on mature and early senescent leaves. Summer aphids from H. vulgare grew as fast on this host as spring aphids did on flush leaves of P. padus. Sexual females grew more slowly than other generations and nearly as well on mature as on early senescent P. padus leaves. As judged by aphid growth and phloem nitrogen quality, P. padus during spring equals the summer host H. vulgare. However, the lower growth rates of R. padi on mature and senescent leaves of P. padus appear only loosely correlated with phloem amino acid concentrations. Therefore, factors influencing aphid nutrition, or ecology, other than seasonal changes in phloem sap amino acid concentration may explain the existence of host alternation in R. padi.