Abstract
A new Beauveria bassiana isolate, showing high activity against Musca domestica L. (Diptera: Muscidae) adults (mortality-100.0%), larvae (mortality-72.3%) and pupae (Infection in emerged flies-96.7%) was used. The isolate was subjected to a combinational approach towards selection of process parameters for its growth optimization. Initial screening of several carbon and nitrogen sources revealed glucose and NaNO3 as the most suitable source for optimal biomass and spore production. Further, optimization through Placket-Burman and a 2(5) full factorial central composite design revealed highly significant effect of glucose and pH. The optimum composition for maximum biomass yield was (g/l): glucose 28; NaNO3 2.43; KH2PO4 1.32; MgSO4 0.60; and pH 7.00. Glucose concentration showed almost linear relationship with biomass yield, indicating its significant contribution in medium composition for fungal growth. Highly significant interactions were observed between glucose and pH, followed by glucose and NaNO3 concentration.
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