Nutritional growth requirements forButyribacterium methylotrophicum on single carbon substrates and glucose

Abstract

Butyribacterium methylotrophicum, an anaerobic acetogen, obligately required pantothenate for growth on either glucose, CH3OH−CO2, H2−CO2, or carbon monoxide. Growth on glucose but not single carbon substrates was stimulated by lipoate and biotin. Sulfide but not sulfate served as the sole sulfur source for growth. This study established thatB. methylotrophicum was both a true autotroph when grown on H2−CO2 and a unicarbonotroph on CO as the sole carbon and energy source. In addition, the vitamin requirements of this species further suggest its distinctiveness fromEubacterium limosum (Butyribacterium rettgeri).