Abstract
MNNG survival and mutagenesis were studied in Anabaena doliolum Bharadwaja. The survival curves of germinating spores were exponential while those of resting spores and filament-fragments were sigmoidal. Blue mutants with much higher phycocyanin contents than the parent were recovered in varying frequencies; the frequency (1.1%) was the highest with 2 mg/ml MNNG for 15 min. Some of the blue mutants sporulated normally; while others did not. A nonsporulating blue mutant (M16) showed branched filaments in liquid cultures. In nitrogen free medium. M16 had a high frequency (70%) of branched filaments during the early phase of growth. In some filaments, the branch arose from a heterocyst showing three polar nodules. There was no other difference between the parent and the mutant in cell morphology and cellular differentiation. High light intensity adversely affected phycocyanin and chlorophyll a pigments and nitrogen fixation in the mutant. Frequency of mutant branched character appears to beta factor of inorganic nitrogen nutrition.
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