Abstract
An indirect method using glucose-U-C 14 to determine amino acid requirements was applied to fifth instar larvae of the pale western cutworm, Agrotis orthogonia Morr. C 14O 2 was produced after glucose-U-C 14 was injected, indicating that the substrate was metabolized. The amino acids, alanine, aspartic acid, glutamic acid, proline, serine, and cysteic acid, isolated from the larvae contained relatively large quantities of radioactivity and were classed as nutritionally non-essential. Phenylalanine, arginine, lysine, histidine, leucine, isoleucine, and cystine contained little radioactivity and were classed as nutritionally essential. Methionine is probably essential also. The requirement for threonine remains in doubt. Tyrosine was not synthesized appreciably from glucose-U-C 14 but was readily formed from phenylalanine.
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