Abstract
Johnson, R. C. (Fort Detrick, Frederick, Md.) and N. D. Gary. Nutrition of Leptospira pomona. III. Calcium, magnesium, and potassium requirements. J. Bacteriol. 85:983-985. 1963.-The cation requirements for Leptospira pomona Wickard were investigated employing a basal medium prepared with 10% deionized rabbit serum and containing 10(-3)m NH(4)Cl, 5 mug/ml thiamine, and 0.01 m Na(2)HPO(4) buffer (pH 7.4). Rabbit serum was deionized by passage through a column of Amberlite IRC-50 resin. This basal medium did not support growth when either Ca(++) or Mg(++) was added to it. However, when both Ca(++) (3 x 10(-5)m) and Mg(++) (5 x 10(-6)m) were present, growth was obtained. Although an absolute requirement for K(+) could not be demonstrated, an increase in the amount of growth did occur if K(+) (5 x 10(-5)m) was incorporated in addition to Ca(++) and Mg(++). When adequate concentrations of Ca(++) and K(+) were present with a limiting concentration of Mg(++) (less than 5 x 10(-7)m), filamentous cells were observed.
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