Nutrient Stress During Ontogeny Alters Patterns of Resource Allocation in two Species of Horned Beetles.

Abstract

The elaboration of exaggerated, sexually selected weapons and ornaments often comes at a cost to other traits. For instance, by sustaining the growth of an exaggerated weapon during development, shared and limited resources such as morphogens, growth factors, and nutrients may become depleted and limit the size to which other structures can grow. Such interactions are characteristic of resource allocation trade-offs, which can constrain the production of phenotypic variation and bias evolutionary trajectories. Across many species of Onthophagus beetles, males produce extravagant horns that are used as weapons in male-male competition over mates. Previous studies have reported resource allocation trade-offs between horns and both proximally and distally developing structures. However, more recent studies have largely failed to recover these patterns, leading to the hypothesis that trade-offs may manifest only in certain species, populations, or environmental conditions. Here, we investigate (i) patterns of resource allocation into horns, eyes, and genitalia in Onthophagus gazella and O. taurus, and assess (ii) how these patterns of resource allocation are influenced by nutrient stress during larval development. We find that nutrient stress alters patterns of resource allocation within and among traits, but recover a trade-off only in the species that invests most heavily into horn production (O. taurus), and in individuals of that species that invested a disproportionately large or small amount of resources into horn growth. These results suggest that resource allocation trade-offs may not be as prevalent as previously described, and that their presence and magnitude may instead be highly context dependent.