Nutrient medium optimization for hyoscyamine production in diploid and tetraploid Datura stramonium L. hairy root cultures

Abstract

The common effect of NO3 −, KH2PO4 and sucrose on the biosynthesis of hyoscyamine by diploid and tetraploid Datura stramonium L. hairy root cultures were studied. Polynominal regression models were elaborated for the description of hyoscyamine biosynthesis as a consequence of variation of the investigated variables (NO3 − between 31 and 47 mM, and sucrose between 20.00 and 40.00 g/l for diploid and NO3 − between 31 and 47 mM; KH2PO4 between 0.50 and 1.25 mM, and sucrose between 50 and 70 g/l, for tetraploid hairy roots, respectively). Optimization procedures allowed us to establish the optimal concentrations of the investigated variables and to propose the modified MS nutrient media, with 1.10 g/l KNO3, 0.17 g/l KH2PO4, and 40 g/l sucrose, for diploid and with 1.10 g/l KNO3, 0.17 g/l KH2PO4, and 50 g/l sucrose, for tetraploid Datura stramonium L. hairy root cultures. The proposed modified MS media provided optimal hyoscyamine production yield for both investigated hairy root cultures.