Abstract
In vertebrate cells, chromosomes oscillate to precisely align during metaphase. NuSAP as a microtubule associated protein, plays a critical role in stabilizing spindle microtubules during metaphase. However, the function of NuSAP in mitosis is largely unknown. In this study, we investigated the role of NuSAP in chromosome alignment, orientation and oscillation utilizing 3D live-cell imaging, which permits a general population of the centromeres with 3D information of the oscillation. Furthermore, we identified NuSAP as a pivotal regulator for the function of a chromokinesin—Kif22 in chromosome oscillation, and specifically investigated the impact of NuSAP on the polar ejection force generated by Kif22. Our findings revealed that NuSAP largely contributes to the polar ejection force generated by Kif22 (Inusap=0.88) during chromosome movement. Taken together, our study provides new insights into the complicated regulation of chromosome oscillation at metaphase to ensure precise chromosome segregation during cell division.
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