Abstract
The use of nurse tissue, effects of genotype and the frequency of post-thaw transfer on the recovery of cryopreserved embryogenic tissues of Pinus radiata that had been stored for 6 months or 4 years were tested. All of the 60 cell lines tested were successfully recovered from liquid nitrogen, and the majority showed improved post-thaw growth when cultured with nurse tissue. Of the 23 lines stored for 4 years, five recovered only when on the nurse treatment.
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