Abstract
The interest of the image analysis procedure is the time-saving in automated planktonic bacterial counting and sizing, with the possibility of manual visual field control at all times. Bacterial biomass (in number and volume) and bacterial projected area histograms were determined with a microcomputer. Performance limits of image-analysed epifluorescence microscopy were: camera sensitivity, considering the very low fluorescence levels on stained bacteria; pixel-μm conversion factor, and the impossibility of the apparatus distinguishing between bacteria and fluorescent small particles. This method is not of interest for counting sediment bacteria.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
