Abstract
1. 1. A numerical technique is presented for the determination of the specificity of proteolytic enzymes and chemical cleavages. The technique was tested using trypsin as the proteolytic enzyme and reduced and alkylated derivatives of lysozyme and ribonuclease as substrates. 2. 2. The number and charge of the tryptic peptidcs (separated by high-voltage electrophoresis) as well as the number of tryptophanyl or tyrosyl peptides, the linear sequence, and the charge at pH 6.5 of each amino-acid residue of the model protein tested were supplied to the computer. 3. 3. The data obtained from the computer with the aid of the above information were in agreement with the known specificity of trypsin, and the composition of each peptide derived by the computer was identical with those obtained by direct analysis of the peptides eluted from the clectrophoretograms.
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