Numerical simulations of high pressure carbon dioxide fluid fluidized beds

The Hydrogen Induced Cracking (HIC) and Sulfide Stress Cracking (SSC) behaviours of sour service and non-sour service carbon steel API 5L X65 were investigated under high pressure carbon dioxide environments, containing elevated amount of hydrogen sulphide (H2S); the test environments simulated offshore pipelines transporting full-well streams in high carbon dioxide (CO2) environments with elevated H2S concentrations. It was systematically studied under standard NACE condition and high pressure carbon dioxide field condition with variation in other key parameters (temperature, pressure and hydrogen sulfide concentration). The HIC and SSC were tested using a High Pressure and High Temperature (HPHT) Autoclave. The surface cracking morphology was analysed using Scanning Electron Microscopy (SEM), Ultrasonic Technique (UT) and Magnetic Particle (MP). The results showed that no cracks were detected in NACE standard and field-condition SSC tests for both sour service and non-sour services carbon steel. In HIC test, crack was detected on non-sour service carbon steel in NACE standard test while no crack was detected on field condition-based tests for both types of carbon steel.

Combined effect of high pressure carbon dioxide and mild heat treatment on overall quality parameters of watermelon juice

Inactivation of peroxidase and polyphenol oxidase in red beet (Beta vulgaris L.) extract with high pressure carbon dioxide

Computational simulations using a low density ratio-based kinetic theory of granular flow in subcritical water fluidized beds

Microbial inactivation of E. coli cells by a combined PEF–HPCD treatment in a continuous flow system

Inactivation of Escherichia coli O157:H7 by high pressure carbon dioxide combined with nisin in physiological saline, phosphate-buffered saline and carrot juice

Enhanced water extraction with high-pressure carbon dioxide on purple sweet potato pigments: Comparison to traditional aqueous and ethanolic extraction

Effect of high pressure carbon dioxide on the properties of water soluble pectin in peach juice

Extracellular pH decline introduced by high pressure carbon dioxide is a main factor inducing bacteria to enter viable but non-culturable state

6 - Evaluation of Supercritical Fluid Interactions with Polymeric Materials

Inactivation of Saccharomyces cerevisiae in conference pear with high pressure carbon dioxide and effects on pear quality

The effect of high pressure carbon dioxide on the inactivation kinetics and structural alteration of phenylalanine ammonia-lyase from Chinese water chestnut: An investigation using multi-spectroscopy and molecular docking methods

High pressure carbon dioxide treatment for fresh-cut carrot slices

Comparative study on cloudy apple juice qualities from apple slices treated by high pressure carbon dioxide and mild heat

High pressure carbon dioxide (HPCD) could cause sublethally injured cells (SICs), which may cause food poisoning and spoilage during food storage and limit its application. Therefore, the formation of SICs of Escherichia coli O157:H7 was investigated by isobaric tag for relative and absolute quantification (iTRAQ) proteomic methods in this study for better controlling the SICs induced by HPCD. A total of 2,446 proteins was identified by iTRAQ, of which 93 and 29 were significantly differentially expressed in the SICs compared with live control cells (CKL) and dead control cells (CKD), respectively. Among the 93 differentially expressed proteins (DEP) in the SICs compared with CKL, 65 proteins showed down-regulation and 28 showed up-regulation. According to the comprehensive proteome coverage analysis, the SICs survived under HPCD by reducing carbohydrate decomposing, lipid transport and metabolism, amino acid transport and metabolism, transcription and translation, DNA replication and repair. Besides, the SICs showed stress response, DNA damage response and an increased carbohydrate transport, peptidoglycan synthesis and disulfide bond formation to HPCD. Among the 29 DEP in the SICs compared with CKD, 12 proteins showed down-regulation and 17 showed up-regulation. According to the comprehensive proteome coverage analysis, the SICs survived under HPCD by accumulation of cell protective agents like carbohydrates and amino acids, and decreasing transcription and translation activities. Results showed that the formation of the SICs with low metabolic activity and high survival ability was a survival strategy for E. coli O157:H7 against HPCD.