Abstract
Publisher Summary This chapter discusses methodological issues related to the processing and evaluation of numerical results from microarray experiments. It illustrates the application of microarray technology to the study of how perturbations of mitochondrial function affect gene expression in yeast. The “wet” steps in spotted DNA microarray methodology including slide washing and coating, polymerase chain reaction (PCR) product manipulation and printing, and mRNA labeling and hybridization are well described. These procedures give excellent results and can be followed essentially without modification. A unique exception to this rule concerns the interval between coating slides and printing. The optimal results can be obtained using slides as soon as possible after coating with poly-L-lysine. The current procedure is to coat slides immediately prior to printing and to steam treat and UV irradiate them immediately after printing. Succinic anhydride treatment may be done shortly before the arrays are used and should be done using materials that are as fresh as possible.
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