Abstract
This paper introduces a sensor for detection of DNA hybridization and investigates its performance by means of computer simulation. A planar transformer with spiral windings is proposed for hybridization detection. In order to detect the occurrence of hybridization, single strand target DNA’s are tagged with magnetic beads. Target DNA’s are then exposed to known single strand probe DNA’s which are immobilized on the surface of a functionalized layer in the proximity of the sensor. The primary winding of the transformer is driven by an AC current source. The voltage at the secondary winding is used for detection. Once the hybridization is occurred, a layer of magnetic material is formed and the coupling between the windings is varied. These variations are reflected into the detecting output voltage. The magnitude of the output voltage is numerically calculated in terms of geometrical and physical parameters and the parameter values resulting in maximum response are derived.
Highlights
Almost all DNA detection techniques are based on the detection of DNA hybridization
This paper introduces a DNA hybridization detection sensor that uses magnetic beads attached to DNA strands as detectable particles
As described in the previous section, the output voltage represents the amount of magnetic beads accumulated above the coils
Summary
Almost all DNA detection techniques are based on the detection of DNA hybridization. Each nucleotide could be one of four chemical constituents called bases. These four bases are Adenine (A), Thymine (T), Guanine (G), and Cytosine (C). Adenine is complementary to Thymine and Guanine is complementary to Cytosine. Binding of two complementary strand of DNA which leads to construction of DNA doublestrand helix is called hybridization of DNA. Bindings between DNA base pairs are very stable in room and body temperatures. Increasing the temperature to around 84 C breaks these bonds and make the DNA single-stranded. This is known as denaturing the DNA
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