Abstract
Microfluidic impedance cytometry is a label-free approach for high-throughput analysis of particles and cells. It is based on the characterization of the dielectric properties of single particles as they flow through a microchannel with integrated electrodes. However, the measured signal depends not only on the intrinsic particle properties, but also on the particle trajectory through the measuring region, thus challenging the resolution and accuracy of the technique. In this work we show via simulation that this issue can be overcome without resorting to particle focusing, by means of a straightforward modification of the wiring scheme for the most typical and widely used microfluidic impedance chip.
Highlights
Microfluidic impedance cytometry is a label-free approach for high-throughput analysis of particles and cells
In this work we show via simulation that this issue can be overcome without resorting to particle focusing, by means of a straightforward modification of the wiring scheme for the most typical and widely used microfluidic impedance chip
Microfluidic impedance cytometry is a label-free technique for analysing single particles and cells at high throughput [1,2]
Summary
Microfluidic impedance cytometry is a label-free technique for analysing single particles and cells at high throughput [1,2] It is used in different biological assays, including particle sizing and counting, cell phenotyping, and disease diagnostics (e.g., [3,4,5,6]). Like in a Coulter volume measurement, the recorded signal depends on the intrinsic properties of the particle, and on its trajectory through the channel [8]. This is due to the non-uniformity of the electric field in the sensing region, and produces blurring of measured particle properties [9,10,11], challenging the accuracy and the resolution of the technique
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