Abstract

Summary Two hundred and seventy-three strains of coryneform bacteria mainly isolated from activated sludge were tested for 135 physiological characters. In addition, one hundred and ten named reference strains were included for comparison. The data were subjected to numerical analysis. The results showed that the strains clustered into 5 groups, each of which consisted of several subgroups. The isolates were identified as members of the genera Arthrobacter, Nocardia, Mycobacterium, Rhodococcus, Microbacterium, Cellulomonas, Curtobacterium and Corynebacterium . The bacterial flora of the activated sludge from dairy sewage mainly consisted of strains of the genera Corynebacterium, Microbacterium and Rbodococcus . That of sludge from municipal sewage predominantly comprised strains of the genera Arthrobacter, Corynebacterium and Microbacterium . Twenty-five of the tests used were found to be particularly useful in discriminating between the main clusters. When the strains were retested and classified on the basis of these tests, the resultant groupings were very similar to those obtained in the full analysis.

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