Number of trials and data structure affect the number and components of muscle synergies in upper-limb reaching movements

Abstract

Objective. Due to the variability of human movements, muscle activations vary among trials and subjects. However, few studies investigated how data organization methods for addressing variability impact the extracted muscle synergies. Approach. Fifteen healthy subjects performed a large set of upper limb multi-directional point-to-point reaching movements. Then, the study extracted muscle synergies under different data settings and investigated how data structure prior to synergy extraction, namely concatenation, averaging, and single trial, the number of considered trials, and the number of reaching directions affected the number and components of muscle synergies. Main results. The results showed that the number and components of synergies were significantly affected by the data structure. The concatenation method identified the highest number of synergies, and the averaging method usually found a smaller number of synergies. When the concatenated trials or reaching directions was lower than a minimum value, the number of synergies increased with the increase of the number of trials or reaching directions; however, when the number of trials or reaching directions reached a threshold, the number of synergies was usually constant or with less variation even when novel directions and trials were added. Similarity analysis also showed a slight increase when the number of trials or reaching directions was lower than a threshold. This study recommends that at least five trials and four reaching directions and the concatenation method are considered in muscle synergies analysis during upper limb tasks. Significance. This study makes the researchers focus on the variability analysis induced by the diseases rather than the techniques applied for synergies analysis and promotes applications of muscle synergies in clinical scenarios.