Abstract
Heterotopic grafting sites can be useful in producing oocytes for in vitro Fertilization, therefore, maximising the oocyte yield from the graft by gonadotrophin stimulation would be advantageous. The aim of this study was to investigate the number and quality of oocytes collected from heterotopic autografted ovary after Pregnant Mare Serum Gonadothropin (PMSG) induction. Graft recipients were treated either with or without PMSG stimulation 48 hours prior to graft collection. Ovarian tissue from four weeks old mice (DDY strain) were autotransplanted under the kidney capsule of the same ovariectomized mice and the oocytes were collected 21 days after autotransplantation. The results showed that the average number of oocytes collected from autografted ovaries without PMSG induction were 9.0. ± 2.8 not significantly different with those received PMSG induction, 10.9 ± 5.1. The percentage of matured and fertilized oocytes and the developed embryos from the autografted ovaries without PMSG induction were 52.4, 33.4, and 26.0%, respectively not significantly different with those received PMSG induction, 53.2, 35.1, and 29.9%, respectively. The number of oocytes and the capacity to matured, fertilized and developed were significantly lower (P
Highlights
Ovary as the primary organ in female reproductive system has two main roles i.e. for producing oocyte and synthesizing essential hormones for follicles development, estrous cyclicity and maintenance of the reproductive tract (Hirshfield 1991)
Ovarian grafts were successfully recovered from all graft recipients in both without and with Pregnant Mare Serum Gonadotropin (PMSG) stimulation, and showed of fully antral follicles development (Figure 1a,b)
The number of oocytes recovered from the fully antral follicles of heterotopic grafted ovaries with and without PSMG induction was not significantly different; this showed that PMSG induction does not significantly increase the oocyte yield from ovarian grafts under the kidney capsule
Summary
Ovary as the primary organ in female reproductive system has two main roles i.e. for producing oocyte and synthesizing essential hormones for follicles development, estrous cyclicity and maintenance of the reproductive tract (Hirshfield 1991). At the time of birth, cortex of mammalian ovary consist of abundant of primordial follicles as oocytes resources; those will develop during puberty (Fortune 1994). Ovaries collected from slaughter house or from immediately dead animal contain follicles in various development stages. These follicles are the oocytes resources used for embryo in vitro production (Gunasena et al 1998; Shaw et al 2000; Paris et al 2004). This ovarian follicles will developed to produce development competence oocytes using in vitro culture and or grafting techniques (Eppig et al 2000; Paris et al 2004, 2009)
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