Numb inhibits proliferation and migration of human bladder cancer cell line BIU-87 by negative modulating CXCR4

Abstract

Objective To study the effect of Numb gene on CXCR4 expression and proliferation and migration of bladder cancer. Methods Human bladder cancer cells were divided into 3 groups: experimental group (transfected with Numb-ORF plasmid), negative-control group (transfected with blank vector), and blank-control group (no DNA transfected in). The expressions of Numb and CXCR4 were detected by real-time PCR and Western blotting respectively. Cell proliferation and migration were measured by MTS and Transwell assay respectively. Results Numb expressions in experimental group, negative-control group and blank-control group were 31.044±3.350, 4.401±0.567 and 4.287±0.341 respectively, with a statistical significance (F=183.418, P=0.000). CXCR4 levels in experimental group, negative-control group and blank-control group were 0.344±0.167, 0.996±0.148 and 1.010±0.106 respectively, with a statistical significance (F=21.355, P=0.002). In MTS assay, the absorb values in experimental group, negative-control group and blank-control group were 0.615±0.057, 0.987±0.063 and 0.957±0.066, with a statistical difference (F=33.210, P=0.001). In Transwell assay, the numbers of migratory cells in experimental group, negative-control group and blank-control group were 164.667±19.858, 670.133±38.760 and 667.533±27.610, with a statistical significance (F=286.788, P=0.000). Conclusion Overexpression of Numb can suppress the ability of proliferation and migration of the BIU-87 cells through down-regulation of CXCR4 expression in human bladder cancer. Key words: Urinary bladder neoplasms; Receptors, CXCR4; Cell proliferation; Cell migration assays